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Image Search Results
Journal: Circulation
Article Title: Physical Exercise Prevents Cellular Senescence in Circulating Leukocytes and in the Vessel Wall
doi: 10.1161/circulationaha.109.861005
Figure Lengend Snippet: Figure 4. Exercise has no effects in eNOS/ mice, and eNOS function is not impaired in TERT/ mice. A through C, Training effects on aortic murine TERT activity and protein expression of TRF2 and p53 in eNOS/ (B6.129/P2-Nos3) mice. Standardiza- tion for GAPDH; n8. D, cGMP in TERT/ mice and wild-type controls; n5. E, Effects of 3 weeks of running exercise on pro- tein expression of phospho-eNOS (peNOS)/eNOS in TERT/ mice and wild-type controls (WT) standardized for GAPDH; n6. ***P0.001. F, Endothelium-dependent (carbachol-induced) vasodilation of isolated aortic rings; regression analysis: P0.037, wild-type runner vs wild-type control; P0.028, TERT runner vs TERT control. G, Endothelium-independent (nitroglyc- erin-induced) vasorelaxation in sedentary and exercised wild- type, TERT/, and eNOS/ mice expressed as percent of max- imal phenylephrine (PE) -induced vasoconstriction (PNS for all groups); n5.
Article Snippet: Proteins were transferred to nitrocellulose membranes (Biorad 162-0112), blocked with 5% dry milk for 30 minutes and exposed to rabbit polyclonal IgG TRF2 (H-300 Santa Cruz, sc- 9143, dilution 1:200), mouse monoclonal IgG P16 (F-12 Santa Cruz, sc-1661, dilution 1:250), 4 rabbit polyclonal IgG anti-P53 (FL-393 Santa Cruz, sc-6243, dilution 1:500 in dry milk 1%), mouse monoclonal IgG Chk2 (A-11 Santa Cruz, sc-17747, dilution 1:1000 in dry milk 1%) and mouse monoclonal IgG GAPDH (6C5 Santa Cruz, sc-32233, dilution 1:1000),
Techniques: Activity Assay, Expressing, Isolation, Control
Journal: Circulation
Article Title: Physical Exercise Prevents Cellular Senescence in Circulating Leukocytes and in the Vessel Wall
doi: 10.1161/circulationaha.109.861005
Figure Lengend Snippet: Figure 5. Exercise protects mice against stress-induced aortic endothelial apoptosis. Endothelial apoptosis of the thoracic aorta was measured by hairpin oligonucleotide assays in C57/ Bl6, TERT/, and eNOS/ mice. A, Representative images showing dark brown peroxidase staining of apoptotic nuclei in the aorta of sedentarylipopolysaccharide (LPS) and runningLPS mice (400 magnification). B, Effect of LPS treat- ment (120 mg IP for 48 hours) in sedentary vs running wild-type, TERT/, or eNOS/ mice vs vehicle-treated controls (Co). Box plots represent median and 25% to 75% percentiles; whiskers represent range; n4 to 5 per group. ***P0.001 vs untreated control mice; ###P0.001 vs LPS-treated sedentary control mice.
Article Snippet: Proteins were transferred to nitrocellulose membranes (Biorad 162-0112), blocked with 5% dry milk for 30 minutes and exposed to rabbit polyclonal IgG TRF2 (H-300 Santa Cruz, sc- 9143, dilution 1:200), mouse monoclonal IgG P16 (F-12 Santa Cruz, sc-1661, dilution 1:250), 4 rabbit polyclonal IgG anti-P53 (FL-393 Santa Cruz, sc-6243, dilution 1:500 in dry milk 1%), mouse monoclonal IgG Chk2 (A-11 Santa Cruz, sc-17747, dilution 1:1000 in dry milk 1%) and mouse monoclonal IgG GAPDH (6C5 Santa Cruz, sc-32233, dilution 1:1000),
Techniques: Staining, Control